| 2024 | Group Therapy | — |
| 2023 | Doctor Who: 60th Anniversary Specials | The Toymaker (archive footage) |
| 2023 | Billion Dollar Babies: The True Story of the Cabbage Patch Kids | Narrator (voice) |
| 2023 | Handsome Podcast (serie) | Self |
| 2023 | Cenando con Drags (serie) | Self - Judge |
| 2022 | Desparejado (serie) | Michael Lawson |
| 2022 | Cómo conocí a tu padre (serie) | Barney Stinson |
| 2021 | Matrix Resurrections | The Analyst |
| 2021 | Navidad en 8 bits | Adult Jake Doyle / Narrator |
| 2021 | Coded: The Hidden Love of J.C. Leyendecker | J.C. Leyendecker (voice) |
| 2020 | Cada cosa en su lugar (serie) | — |
| 2020 | Home Movie: The Princess Bride (serie) | Westley |
| 2020 | Visibilidad: LGTBI en la televisión (serie) | Self - Narrator (voice) |
| 2019 | Epcot International Festival of the Holidays – Candlelight Processional | Narrator |
| 2019 | Star Wars: Galaxy's Edge - Adventure Awaits | Self - Host |
| 2019 | El programa de Kelly Clarkson (serie) | Self |
| 2019 | Padrazos | Self |
| 2018 | Mickey’s 90th Spectacular | Self |
| 2018 | Genius Junior (serie) | Host |
| 2017 | Carpool Karaoke: La serie (serie) | Self |
| 2017 | Una serie de catastróficas desdichas (serie) | Count Olaf |
| 2016 | Neil Patrick Harris: Circus Awesomeus | Host / Himself |
| 2015 | Best Time Ever with Neil Patrick Harris (serie) | Himself |
| 2015 | In Their Own Words: Jim Henson | Self - Actor |
| 2015 | Celebrity Family Feud (serie) | Self |
| 2014 | Perdida | Desi Collings |
| 2014 | Mil maneras de morder el polvo | Foy |
| 2014 | 73 preguntas (serie) | Self |
| 2014 | Late Night with Seth Meyers (serie) | Self |
| 2014 | The Tonight Show Starring Jimmy Fallon (serie) | Self |
| 2014 | Off Camera with Sam Jones (serie) | Self |
| 2014 | Steve's First Bath | Steve (voice) |
| 2014 | Attack of the 50-foot Gummi Bear | Steve the Monkey (voice) |
| 2013 | Stephen Fry: Out There (serie) | Himself |
| 2013 | Lluvia de albóndigas 2 | Steve (voice) |
| 2013 | Beat Bobby Flay (serie) | Self |
| 2013 | Los pitufos 2 | Patrick Winslow |
| 2013 | Lejos del armario | Himself |
| 2012 | Neil's Puppet Dreams (serie) | Neil Patrick Harris |
| 2012 | A Snow White Christmas: Opening Night | The Magic Mirror |
| 2012 | Finding Your Roots (serie) | Self |
| 2012 | The Penguins of Madagascar: Operation Blowhole | Dr. Blowhole (voice) |
| 2011 | Billy on the Street (serie) | Self |
| 2011 | Dos colgaos muy fumaos en Navidad | Neil Patrick Harris |
| 2011 | Brain Games (serie) | Himself - Host |
| 2011 | American Horror Story (serie) | Chester Creb |
| 2011 | Los pitufos | Patrick Winslow |
| 2011 | Hamish and Andy's Gap Year (serie) | Self |
| 2011 | Company | Robert |
| 2011 | El corazón de la bestia | Will Fratalli |
| 2010 | The Best and the Brightest | Jeff |
| 2010 | Como perros y gatos: La venganza de Kitty Galore | Lou (voice) |
| 2010 | Batman: Capucha Roja | Dick Grayson / Nightwing (voice) |
| 2010 | Through a Dog's Eyes | Narrator |
| 2010 | Hora de aventuras (serie) | Prince Gumball |
| 2010 | Dracula's Daughter vs. the Space Brains | Dan |
| 2010 | A First Look at Batman: Under the Red Hood | Self |
| 2009 | Yes Virginia | Dr. Philip O'Hanlon (voice) |
| 2009 | Watch What Happens Live with Andy Cohen (serie) | Invitado especial |
| 2009 | Top Chef Masters (serie) | Himself - Guest Judge |
| 2009 | Glee (serie) | Bryan Ryan |
| 2009 | Late Night with Jimmy Fallon (serie) | Self |
| 2009 | RuPaul: Reinas del drag (serie) | Self - Guest Judge |
| 2008 | Los pingüinos de Madagascar (serie) | Dr. Blowhole (voice) |
| 2008 | El intrépido Batman (serie) | Music Meister (voice) |
| 2008 | Dr. Horrible's Sing-Along Blog (serie) | Dr. Horrible |
| 2008 | Commentary! The Musical | — |
| 2008 | Dr. Horrible's Sing-Along Blog | Doctor Horrible |
| 2008 | Dos colgaos muy fumaos: Fuga de Guantánamo | Neil Patrick Harris |
| 2008 | La Liga de la Justicia: La nueva frontera | Barry Allen / The Flash (voice) |
| 2007 | The Making of Dr. Horrible's Sing-Along Blog | Himself |
| 2007 | Eloise Goes to School | — |
| 2007 | The Graham Norton Show (serie) | Self |
| 2006 | Me, Eloise (serie) | Philip (voice) |
| 2006 | Rachael Ray (serie) | — |
| 2006 | The Megan Mullally Show (serie) | Invitado especial |
| 2005 | Milagros de amor | Nathan Andrews |
| 2005 | The Reichen Show (serie) | Invitado especial |
| 2005 | The Colbert Report (serie) | — |
| 2005 | Cómo conocí a vuestra madre (serie) | Barney Stinson |
| 2005 | The Golden Blaze | The Comic Shop Owner (voice) |
| 2005 | Numb3rs (serie) | — |
| 2005 | The Late Late Show with Craig Ferguson (serie) | Self |
| 2004 | The Tony Danza Show (serie) | Invitado especial |
| 2004 | Jack & Bobby (serie) | — |
| 2004 | Dos colgaos muy fumaos | Neil Patrick Harris |
| 2003 | The Ellen DeGeneres Show (serie) | Self |
| 2003 | Las Increíbles Aventuras de Spiderman (serie) | Peter Parker / Spider-Man (voice) |
| 2003 | Cazados (serie) | Self |
| 2003 | Jimmy Kimmel Live! (serie) | — |
| 2002 | Boomtown (serie) | Peter Corman |
| 2002 | Undercover Brother (El hermano secreto) | Lance |
| 2002 | Death from Above: The Making of 'Starship Troopers' | Himself |
| 2002 | The Making of Sweeney Todd at the San Francisco Symphony | Self |
| 2002 | The Mesmerist | — |
| 2001 | La liga de la justicia (serie) | Ray Thompson (voice) |
| 2001 | Live from Lincoln Center: Sweeney Todd: The Demon Barber of Fleet Street - In Concert with the New York Philharmonic | Tobias |
| 2001 | Ley y orden: Acción criminal (serie) | John Tagman |
| 2001 | Tarzán: La serie animada (serie) | Moyo (voice) |
| 2000 | Ed (serie) | Joe Baxter |
| 2000 | Descarga estática (serie) | Replay / Johnny Morrow (voice) |
| 2000 | Son of the Beach (serie) | — |
| 1999 | The Early Show (serie) | Self |
| 1999 | Stark, loco de atar (serie) | Henry McNeeley |
| 1999 | Juana de Arco (Christian Duguay) (serie) | The Dauphin |
| 1999 | Padre de familia (serie) | Barney Stinson (voice) |
| 1998 | Un regalo de Navidad | Will Martin |
| 1998 | Promesas incumplidas | Roger Martin |
| 1997 | Starship Troopers: Las brigadas del espacio | Colonel Carl Jenkins |
| 1997 | The View (serie) | Self |
| 1996 | The Daily Show (serie) | Self |
| 1995 | Legacy of Sin: The William Coit Story | William Coit |
| 1995 | The Man in the Attic | Edward Broder |
| 1995 | My Antonia | Jimmy Burden |
| 1995 | Más allá del límite (serie) | Howie Morrison |
| 1995 | Not Our Son | Paul Keller |
| 1995 | Animal Room | Arnold Mosk |
| 1994 | Tocados por un ángel (serie) | Jonas |
| 1994 | Infierno bajo la nieve | Jim Stolpa |
| 1993 | Una familia deshecha | Brian Hannigan |
| 1993 | Late Night with Conan O'Brien (serie) | Invitado especial |
| 1993 | Homicidio (serie) | — |
| 1992 | Capitol Critters (serie) | Max (voice) |
| 1991 | Stranger in the Family | Steve Thompson |
| 1991 | The Best of Disney: 50 Years of Magic | Self |
| 1990 | El Capitán Planeta y los Planetarios (serie) | Todd Andrews |
| 1990 | Blossom (serie) | — |
| 1989 | Los Simpson (serie) | Neil Patrick Harris (voice) |
| 1989 | Un médico precoz (serie) | Doogie Howser |
| 1989 | A través del tiempo (serie) | Mike Hammond |
| 1989 | B.L. Stryker (serie) | Buder |
| 1989 | Home Fires Burning | Lonnie Tibbettes |
| 1988 | Purple People Eater | Billy Johnson |
| 1988 | Pasión fatal | Danny Harland |
| 1988 | El desafío de una mujer | David Hart |
| 1988 | LIVE with Kelly and Mark (serie) | Invitado especial |
| 1985 | Larry King Live (serie) | Self |
| 1984 | Jeopardy! (serie) | — |
| 1981 | Entertainment Tonight (serie) | Self |
| 1975 | Saturday Night Live (serie) | Self - Host |
| 1953 | The Oscars (serie) | Host |
| 1951 | Hallmark Hall of Fame (serie) | Lonnie |
